Analytical Chemistry with Laboratory
Lectures: 8 h
Laboratory: 10 h
Theoretical basis of chromatographic separations: thermodynamic aspects, kinetic theory of peak broadening and Van Deemter’s Equation. Chromatographic resolution equation.
Gas chromatography: types of inlets, types of columns, instrumental variables affecting gas chromatographic separation.
Technical specifications of detectors that can be used in gas chromatography. Iphenated mass spectrometry techniques with gas chromatography: electron ionization and chemical ionization techniques. Quadrupole analyzers.
Identification of a chromatographic peak: retention time and retention indexes. Quantitative analysis methods in chromatography: internal standard method, standard addition method, area normalization method.
Laboratory: analysis of a fragrance blend: identification and quantification of the specific components.
Prof. Massimo Del Bubba, Dr. Lapo Renai
Department of Chemistry “Ugo Schiff”
University of Florence, Italy
email: massimo.delbubba (at) unifi.it | lapo.renai (at) unifi.it
tel.: + 39 055 4573326